• 18-7 Kubec R, Štefanová I, Moos M, Urajová P, Kuzma M, Zápal J (2018), Allithiolanes – nine groups of a newly discovered family of sulfur compounds responsible for the bitter off-taste of processed onion. Journal of agriculture and food chemistry DOI: 10.1021/acs.jafc.8b03118.
  • 18-6 Rozsypal J, Moos M, Goto SG (2018), Cold acclimation increases cold tolerance independently of diapause programing in the bean bug, Riptortus pedestris. Bulletin of Entomological Research 108/4 (2018) 83-92 DOI: 10.1017/S0007485317001006.
  • 18-5 Rozsypal J, Moos M, Šimek P, Košťál V (2018), Thermal analysis of ice and glass transitions in insects that do and do not survive freezing. Journal of Experimental Biology (2018) 221: jeb.170464 DOI: 10.1242/jeb.170464.
  • 18-4 Hušek P, Švagera Z, Hanzlíková D, Karlínová I, Řimnáčová L, Zahradníčková H, Šimek P (2018), GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol; Georgios A. Theodoridis et al. (eds.), Metabolic Profiling: Methods and Protocols, Methods in Molecular Biology 2018, vol. 1738, pages 159-181, Chapter 11. © Springer Science+Business Media, LLC, part of Springer Nature 2018 DOI: org/10.1007/978-1-4939-7643-0_11.
  • 18-3 Štětina T, Hůla P, Moos M, (2018) Recovery from supercooling, freezing, and cryopreservation stress in larvae of the drosophilid fly, Chymomyza costata. Scientific Reports 8 (2018) 4414 DOI: 10.1038/s41598-018-22757-0.
  • 18-2 Volf M, Segar ST, Miller SE, Isua B, Sisol M, Aubona G, Šimek P, Moos M, Salminen J-P, Zima J, Jadranka Rota J, Weiblen GD, Wossa S, Novotny V (2018), Community structure of insect herbivores is driven by defensive traits with contrasting evolutionary dynamics: the role of trait escalation and divergence in Ficus. Ecology Letters 21 (2018) 83–92 DOI: 10.1111/ele.12875.
  • 18-1 Lehmann P, Pruisscher P, Košťál V, Moos M, Šimek P, Nylin S, Agren R, Väremo L, Wiklund C, Wheat CW, Gotthard K (2018), Metabolome dynamics of diapause in the butterfly Pieris napi: distinguishing maintenance, termination and post-diapause phases. Journal of Experimental Biology (2018) 221: jeb169508 DOI: 10.1242/jeb.169508.



  • 17-05 Řimnáčová L, Biology Centre CAS, New procedures in the metabolomic analysis of biofluids, PhD Thesis, Charles University, Faculty of Science, Prague 2017.
  • 17-04 Vrba P, Nedvěd O, Zahradníčková H, Konvička M (2017), More complex than expected: Cold hardiness and the concentration of cryoprotectants in overwintering larvae of five Erebia butterflies; European Journal of Entomology 114 (2017) 470-480 DOI: 10.14411/eje.2017.060.
  • 17-03 Vrba P, Nedvěd O, Zahradníčková H, Konvička M (2017), Temporal plasticity in cold hardiness and cryoprotectant contents in northern versus temperate Colias butterfies (Lepidoptera: Pieridae). Cryoletters 38 (2017) 330-338.
  • 17-02 Gäde G, Šimek P, Marco HG (2017), The African froghopper Ptyelus flavescens (suborder: Cicadomorpha) contains two novel and one known peptides of the adipokinetic hormone (AKH) family: structure, function and comparison with aphid AKH (suborder: Sternorrhyncha).  Amino Acids 49/10 (2017), 1679-1690, DOI 10.1007/s00726-017-2461-y.
  • 17-01 Tomčala A, Kyselová V, Schneedorferová I, Opekarová I, Moos M, Urajová P, Kručinská J, Oborník M (2017), Separation and identification of lipids in the photosynthetic cousins of Apicomplexa Chromera velia and Vitrella brassicaformis. Journal of Separation Science  40/17 (2017) 3402-3413, DOI: 10.1002/jssc.201700171.



  • 16-11 Moos M.Šimek P., Petrus K., Hušek P, Robotic Sample Preparation Workstation  MetaboAuto® – PGL HFBCF MXP-U. Applied Technology.
  • 16-10 Gäde G, Šimek P, Marco HG (2016), Novel members of the adipokinetic hormone family in beetles of the superfamily Scarabaeoidea. Amino Acids 48 (2016), 2785-2798, DOI: 10.1007/s00726-016-2134-0.
  • 16-09 Boardman L, Sørensen J G, Košťál V, Šimek P, Terblanche JS (2016), Chilling slows anaerobic metabolism to improve anoxia tolerance of insects. Metabolomics 12 (2016), 176-184, DOI: 10.1007/s11306-016-1119-1.
  • 16-08 Colinet H, Renault D, Javal M, Berková P, Šimek P, Košťál V (2016), Uncovering the benefits of fluctuating thermal regimes on cold tolerance of drosophila flies by combined metabolomic and lipidomic approach. BBA – Molecular and Cell Biology of Lipids 1861 (2016), 1736-1745.
  • 16-07 Boardman L, Sørensen JG, Košťál V, Šimek P and Terblanche JS (2016), Cold tolerance is unaffected by oxygen availability despite changes in anaerobic metabolism. Scientific Reports 6 (2016), 32856, DOI: 10.1038/srep32856.
  • 16-06 Košťál V, Korbelová J, Štětina T, Poupardin R, Colinet H, Zahradníčková H, Opekarová I, Moos M and Šimek P (2016), Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster. Scientific Reports 6 (2016), DOI: 10.1038/srep32346.
  • 16-05 Košťál V, Korbelová J, Poupardin R, Moos M and Šimek P (2016), Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster. Scientific Reports 6 (2016), 32346, DOI: 10.1038/srep32346.
  • 16-04 Mollaei M, Izadi H, Šimek P and Košťál V (2016), Overwintering biology and limits of cold tolerance in larvae of pistachio twig borer, Kermania pistaciella. Bulletin of Entomological Research 106 (2016), 538–545. DOI: 10.1017/S0007485316000237.
  • 16-03 Gäde G, Šimek P, Marco HG (2016), Identification and bioactivity evaluation of the first neuropeptide from the lesser‑known insect order Embioptera (webspinner). Amino Acids 48/7 (2016), 1677-1684, DOI: 10.1007/s00726-016-2229-9.
  • 16-02 Dean CAE, Nicolas M. Teets NM, Košťál V, Šimek P, Denlinger DL (2016), Enhanced stress responses and metabolic adjustments linked to diapause and onset of migration in the large milkweed bug Oncopeltus fasciatus. Physiological Entomology 41 (2016), 152–161. DOI: 10.1111/phen.12140.
  • 16-01 Hušek P, Švagera Z, Hanzlíková D, Řimnáčová L, Zahradníčková H, Opekarová I, Šimek P (2016), Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry. Journal of Chromatography A 1443 (2016), 211-232. DOI: 10.1016/j.chroma.2016.03.019.



  • 14-08 Vrba P, Dolek M, Nedvěd O, Zahradníčková H, Cerrato C, Konvička M (2014), Overwintering of the boreal butterfly Colias palaeno in Central Europe. CryoLetters 35 (2014), 247-254.
  • 14-07 Paroulek M, Sláma K (2014), Production of the sesquiterpenoid, juvenile hormone-1 (JH-I), and of vitamin E in the accessory sexual (colleterial) glands of adult male moths, Hyalophora cecropia (Linnaeus, 1758), (Lepidoptera: Saturniidae). Life-The Excitement of Biology 2 (2014), 102-124. DOI: 10.9784/LEB2(2)Paroulek.01.
  • 14-06 Košťál V, Miklas B, Doležal P, Rozsypal J, Zahradníčková H (2014), Physiology of cold tolerance in the bark beetle, Pityogenes chalcographus and its overwintering in spruce stands. Journal of Insect Physiology 63 (2014), 62-70. DOI: 10.1016/j.jinsphys.2014.02.007.
  • 14-05 Kalová H, Janečková B, Verner M, Šimek P, Šintaj A, Šťastná H, Petr P (2014), Some biochemical, organoleptic and nutraceutical properties of wine with special emphasis on Moldovan wines: wine as medicamentum. Prevence úrazů, otrav a násilí 10 (2014), 38–52.
  • 14-04 Rozsypal J, Košťál V, Berková P, Zahradníčková H, Šimek P (2014), Seasonal changes in the composition of storage and membrane lipids in overwintering larvae of the codling moth, Cydia pomonella. Journal of Thermal Biology 45 (2014), 124-133. DOI: 10.1016/j.therbio.2014.08.011
  • 14-03 Marco HG, Šimek P, Gäde G (2014), Adipokinetic hormones of the two extant apterygotan insect orders, Archaeognatha and Zygentoma. Journal of Insect Physiology 60 (2014,) 17-24. DOI: 10.1016/j.jinsphys.2013.11.002.
  • 14-03 Řimnáčová L, Hušek P, Šimek P (2014), A new method for immediate derivatization of hydroxyl groups by fluoroalkyl chloroformates and its application for the determination of sterols and tocopherols in human serum and amniotic fluid by gas chromatography–mass spectrometry. Journal of Chromatography A, 1339 (2014), 154-167. DOI.org/10.1016/j.chroma.2014.03.007
  • 14-01 Zahradníčková H, Tomčala A, Berková P, Schneedorferová I, Okrouhlík J, Šimek P, Hodková M (2014), Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: application to insects. Journal of Separation Science 37 (2014), 2062-2068. DOI: 10.1002/jssc.201400113.


  • 13-05 Heather HG, Šimek P, Gäde G (2013), Novel adipokinetic hormones of the kissing bug Rhodnius prolixus, Triatoma infestans, Dipetalogaster maxima and Panstrongylus megist Peptides 41 (2013), 24-30. http://dx.doi.org/10.1016/j.peptides.2012.09.032.
  • 13-04 Košťál V, Urban T, Řimnáčová L, Berková P, Šimek P (2013), Seasonal changes in minor membrane phospholipid classes, sterols and tocopherols in overwintering insect, Pyrrhocoris apterus. Journal of Insect Physiology 59 (2013), 934-941.
  • 13-03 Gäde G, Šimek P, Clark KD, Marco HG (2013), Five functional adipokinetic peptides expressed in the corpus cardiacum of the moth genus Hippotion (Lepidoptera, Shingidae). Regulatory Peptides 184 (2012), 85-95.
  • 13-02 Košťál V, Rozsypal J, Pech P, Zahradníčková H, Šimek P (2013), Physiological and biochemical responses to cold and drought in the rock-dwelling pulmonate snail, Chondrina avenacea. Journal of Comparative Physiology B 183 (2012), 749-761.
  • 13-01 Rozsypal J, Košťál V, Zahradníčková H, Šimek P (2013), Overwintering strategy and mechanisms of cold tolerance in the codling moth (Cydia pomonella). PLoS One 8/4 (2013), e61745.


  • 12-12 Vesala L, Salminen TS, Košťál V, Zahradníčková H, Hoikkala A (2012), Myo-inositol as a main metabolite in overwintering flies: seasonal metabolomic profiles and cold stress tolerance in northern drosophilid fly. Journal of Experimental Biology 215 (2012), 2891-2897. DOI:10.1242/jeb.069948.
  • 12-11 Hrouzek P, Kuzma M, Černý J, Novák P, Fišer R, Šimek P, Štys D, Lukešová A, Kopecký J (2012), The cyanobacterial cyclic lipopeptides puwainaphycins F/G are inducing necrosis via cell membrane permeabilization and subsequent unusual act in relocalization. Chemical Research in Toxicology 25 (2012), 1203-1211. DOI: 10.1021/tx300044t.
  • 12-10 Hušek P, Švagera Z, Hanzlíková Z, Šimek P (2012), Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography. Journal of Pharmaceutical and Biomedical Analysis 67-68 (2012), 159-162. DOI: 10.1016/j.jpba.2012.04.027.
  • 12-09 Jedlička P, Steinbauerová V, Šimek P, Zahradníčková H (2012), Functional characterization of the adipokinetic hormone in the pea aphid, Acyrthosiphon pisum. Comparative Biochemistry and Physiology – Part A: Molecular & Integrative Physiology 162 (2012), 51-58. DOI: 10.1016/j.cbpa.2012.02.004.
  • 12-08 Weaver RJ, Marco HG, Šimek P, Audsley N, Clark KD, Gäde G (2012), Adipokinetic hormones (AKHs) of sphingid Lepidoptera, including the identification of a second Manduca sexta Peptides 34 (2012), 44-50. DOI: 10.1016/j.peptides.2012.01.009.
  • 12-07 Švagera Z, Hanzlíková Z, Šimek P, Hušek P (2012), Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography–mass spectrometry. Analytical and Bioanalytical Chemistry 402 (2012), 2953-2963. DOI: 10.1007/s00216-012-5727-y
  • 12-06 Fleischmannova J, Kucerova L, Sandova K, Steinbauerova V, Broz V, Simek P, Zurovec M (2012), Differential response of Drosophila cell lines to extracellular adenosine. Insect Biochemistry & Molecular Biology 42 (2012), 321-331. DOI:10.1016/j.ibmb.2012.01.002.
  • 12-05 Košťál V, Šimek P, Zahradníčková H, Cimlová J, Štětina T (2012), Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism. Proceedings of the National Academy of Sciences USA 109 (2012), 3270-3274. DOI: 10.1073/pnas.1119986109.
  • 12-04 Vranova V, Zahradnickova H, Janous D, Skene KR, Avtar S. Matharu AS, Rejsek K, Formanek P (2012), The significance of D-amino acids in soil, fate and utilization by microbes and plants: review and identification of knowledge gaps. Plant and Soil 354 (2012), 21–39, DOI 10.1007/s11104-011-1059-5.
  • 12-03 Hušek P (2012), Letter to the editor regarding GC–MS with ethyl chloroformate derivatization for comprehensive analysis of metabolites in serum and its application to human uremia. Analytical and Bioanalytical Chemistry 403 (2012), 2758-2786, DOI 10.1007/s00216-011-5615-x.
  • 12-02 Cimlová J, Kružberská P, Švagera Z, Hušek P, Šimek P (2012), In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography–tandem mass spectrometry. Journal of Mass Spectrometry 47 (2012), 294-302. DOI:10.1002/jms.2952.
  • 12-01 Šimek P, Hušek P, Zahradníčková H (2012), Heptafluorobutyl chloroformate – based sample preparation protocol for nonchiral and chiral analysis of amino acids by gas chromatography. Methods in Molecular Biology 828 (2012), 137-152. Alterman N.A., Hutzinger P. eds., Amino Acid Handbook, Chapter 13, Springer Media & Bussiness Media, New York 2012. DOI: 10.1007/978-1-61779-445-2_13.


  • 11-08 Gäde G, Šimek P, Marco HG (2011), An invertebrate [hydroxyproline]-modified neuropeptide: further evidence for a close evolutionary relationship between insect adipokinetic hormone and mammalian gonadotropin hormone family. Biochemical and Biophysical Research Communications 414 (2011), 592-597.
  • 11-07 Košťál V, Korbelová J, Zahradníčková H, Cimlová J, Tomčala A, Šimek P (2011), Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster. PlosOne 6 (2011), e25025.DOI : 10.1371/journal.pone.0025025
  • 11-06 Košťál V, Zahradníčková H, Šimek P (2011), Hyperprolinemic larvae of drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen. Proceedings of the National Academy of Sciences USA 108 (2011), 13041-13046. DOI: 10.1073/pnas.1107060108.
  • 11-05 Košťál V, Doležal P, Rozsypal J, Moravcová M, Zahradníčková H, Šimek P (2011), Physiological and biochemical analysis of overwintering and cold tolerance in two Central, European populations of the spruce bark beetle Ips typographus. Journal of Insect Physiology 57 (2011), 1136-1146. DOI: o10.1016/j.jinsphys.2011.03.011.
  • 11-04 Kubec R, Krejcova P, Simek P, Vaclavík L, Hajslova J, Schraml J (2011), Precursors and formation of pyrithione and other pyridyl-containing sulfur compounds in drumstick onion, Allium stipitatum. Journal of Agricultural and Food Chemistry 59 (2011), 5763-5770. DOI: 10.1021/jf200704n.
  • 11-03 Marco HG, Šimek P, Gäde G (2011), The first decapeptide adipokinetic hormone (AKH) in Heteroptera; a novel AKH from a South African saucer bug, Laccocoris spurcus (Naucoridae, Laccocorinae). Peptides 32 (2011), 454-460.
  • 11-02 Kučerová P, Kubec R, Šimek P, Václavík L, Schraml J (2011), Allium discoloration: The precursor and formation of the red pigment in giant onion (Allium giganteum Regel) and some other subgenus Melanocrommyum species. Journal of Agricultural and Food Chemistry 59 (2011), 1821-1828.
  • 11-01 Gäde G, Šimek P, Fescemyer HW (2011), Adipokinetic hormones provide interference for the phylogeny of Odonata. Journal of Insect Physiology 57 (2011), 174-178.